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The second reaction cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circular dichroism. As drawn in Fig - https://www.slideshare.net/julieanndesalorenz30. 9, the reaction might also involve electron transfer from H4B to the Fe, IIO2 intermediate

l9 As revealed in Fig. 9, this could be since the H4B their explanation extreme acts as an electron sink in a later point in the reaction, so regarding allow NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.